Precisely Matching Your Sorting Needs: A Complete Selection Guide to BORHEE Cell Sorting Magnetic Racks

Before selecting a magnetic rack, it is essential to understand the differences between the two core sorting strategies and their corresponding rack requirements. Positive sorting directly labels the target cells: the bead-labeled target cells are captured by the magnetic rack, which suits scenarios with highly specific, highly expressed targets and places high demands on field strength and gradient design. Negative sorting labels the non-target (impurity) cells instead, requiring excellent field uniformity and multi-tube compatibility so that impurities are thoroughly removed while the untouched target cells remain in the supernatant.

I. Core Sorting Strategies and Rack Matching Principles

Whether positive or negative, rack selection should center on four dimensions: magnetic field performance (strength, uniformity, gradient), sample throughput (single-channel/multi-channel), processing volume (micro/routine/large volume) and experimental scenario (rare cell capture / routine sorting / high-throughput screening). BORHEE addresses each of these dimensions with dedicated product series:

- Positive sorting: prioritize models with high field strength and optimized gradients to ensure fast capture of bead-labeled target cells and higher recovery, especially for rare cells and low-expression targets.

- Negative sorting: prioritize field uniformity and multi-tube compatibility to thoroughly remove non-target cells while avoiding non-specific capture caused by excessive field strength.

- High throughput: choose multi-channel models with consistent field across channels for parallel processing of batch samples.

- Large volume / special samples: choose dedicated models with deep field penetration and large tube compatibility for PBMC, cord blood and other large volume samples.

II. BORHEE Product Series Analysis and Scenario Matching

(1) MAG-XB Series: High-Field Precision Sorting (Core Choice for Negative Sorting)

The MAG-XB series focuses on high-purity, high-precision sorting. Built with NdFeB N52-grade permanent magnets and an optimized field gradient design, it delivers strong, precisely distributed magnetic fields for negative sorting and demanding separation experiments, efficiently capturing bead-labeled target cells - especially rare and sensitive cells.

MAG-XB-1 single-tube rack: pole surface field >9,000 Gs, the strongest in the series; fits 5 mL flow tubes and 1.5-2.0 mL centrifuge tubes; typical sorting time <2 min; recovery and purity both >98% (ideal for rare cell enrichment).

MAG-XB-2 enhanced single-tube rack: field >7,700 Gs with a patented gradient field design that reduces cell clumping and protects viability; sorting time <3 min; recovery >97%, purity >98%. The gradient design also lowers bead residue in negative sorting workflows.

MAG-XB-4 four-channel rack: 4 independent channels, field >7,400 Gs; processes four 5 mL samples simultaneously with <3% field variation between channels and inter-channel recovery CV <2%, improving throughput by ~70% over sequential single-channel operation.

MAG-XB-8 eight-channel rack: 8 optimized positions, field >7,400 Gs with uniformity matching the MAG-XB-4; fits 5 mL centrifuge tubes. Designed for high-throughput negative sorting such as multi-group controlled experiments and small-scale screening.

(2) MAG-MS Series: General-Purpose Sorting (Main Force for Positive Sorting)

The MAG-MS series is built around multi-scenario adaptability and ease of use, with optimized field uniformity and container compatibility. It fits MS-series separation columns, and some models support pole replacement - meeting the impurity-removal demands of negative sorting as well as routine positive sorting, making it a versatile laboratory workhorse.

MAG-MS-1 single-tube general-purpose rack: balanced field design suited to routine positive sorting; fits MS-series columns; sorting purity >90% for standard immune cell sorting. Ideal for small-scale positive sorting such as initial enrichment of peripheral blood mononuclear cells (PBMC).

MAG-MS-8 eight-channel general-purpose rack: 8 parallel channels with excellent field consistency; fits MS-series columns for high-throughput positive sorting - multi-group parallel experiments and batch immune cell sorting such as positive enrichment of mouse splenic lymphocytes, while also supporting large-scale negative sorting.

(3) MAG-LS Series: Large Volume Sample Sorting (Dedicated for Large Volume, Special Scenarios)

The MAG-LS series is optimized for large volume samples with enhanced field penetration depth and fits LS-series separation columns. Its uniform field distribution efficiently processes PBMC, cord blood and other large volume samples, filling the market gap in large volume sorting solutions.

MAG-LS-1 single-tube large volume rack: optimized field penetration for LS-series columns; suits large-scale positive sorting such as label-free enrichment of target cells from cord blood and cell separation from large culture supernatant volumes, ensuring complete capture of bead-cell complexes in large containers.

MAG-LS-4 four-channel large volume rack: 4 independent large volume channels processing four LS-series columns simultaneously, combining large volume with high throughput - e.g. preclinical batch PBMC sorting and biobank sample processing, with guaranteed field uniformity across channels.

III. Core Advantages of BORHEE's Diversified Solutions

Through differentiated design across three series, BORHEE provides a full-scenario solution covering positive enrichment, label-free negative sorting, large volume batch processing and high-throughput parallel experiments. Three core advantages:

- Precise strategy matching: reinforced field strength and gradient for negative sorting (MAG-XB series); optimized uniformity and multi-tube compatibility for positive sorting (MAG-MS and MAG-LS series) - avoiding the limitations of one-size-fits-all products and ensuring high recovery under any strategy.

- Full-scenario throughput coverage: from single tube to 8 channels, from micro centrifuge tubes to 50 mL large volume containers, meeting needs from single samples to batch processing across basic labs, high-throughput screening platforms and preclinical institutions.

- Stable performance and high compatibility: the entire series uses medical-grade epoxy coating, passing ISO 10993 biocompatibility testing; corrosion resistant and easy to clean. Some models are SBS-footprint compliant for automation platforms and compatible with mainstream bead brands, ensuring experimental consistency and reproducibility.

IV. Quick Selection Decision Tree

To simplify selection, match your core requirements with the following logic:

- Define the sorting strategy: negative sorting -> MAG-XB series first; positive sorting -> MAG-MS (routine volume) / MAG-LS (large volume) series.

- Determine sample throughput: single/few samples -> single-tube models (MAG-XB-1/2, MAG-MS-1, MAG-LS-1); batch samples -> multi-channel models (MAG-XB-4/8, MAG-MS-8, MAG-LS-4).

- Match sample volume: routine (≤5 mL) -> MAG-XB and MAG-MS series; large volume (15-50 mL) -> MAG-LS series.

- Refine by requirement: rare cells / highest-purity positive sorting -> MAG-XB-1 (ultra-high field) / MAG-XB-2 (gradient field); routine positive sorting -> MAG-MS series; large volume positive sorting -> MAG-LS series.

Conclusion

The essence of cell sorting is precise matching. With 8 magnetic rack models across the MAG-XB, MAG-MS and MAG-LS series, BORHEE breaks the scenario limitations of single products and achieves full-dimensional coverage from sorting strategy and sample throughput to processing volume. Whether you pursue high-purity positive enrichment, label-free negative sorting or large volume high-throughput processing, BORHEE has a proven solution for your laboratory.